My research career thus far has spanned in vivo whole animal studies to cell and molecular biology in mammalian systems. My training also encompasses experimental and computational biology. My research as a graduate student examined the role of gap junction protein Connexin in regulating lens development and promoting lens differentiation. During this period, I acquired a broad background in Biochemistry and Molecular Biology, as well as membrane cell biology. I also independently carried out computational structural simulation of important membrane proteins, which strengthened the experimental findings in my previous research. In my previous postdoc research, I focused on studying the role of ROS/NAPDH oxidase and related signaling pathways during development of cancer and diabetes. I use an in vitro primary cells model that permits manipulation of various gene expression and examination of signaling pathways involved in pathogenesis of the disease. In vivo cancer mouse model is also used in my research to elucidate further the mechanisms by which/how diabetes/cancer enhances ROS and Nox protein expression and related mechanism. In Dr. Bhat’s Lab I am currently focusing on mechanistic studies of myelination in a rare multisystem genetic disorder, tuberous sclerosis complex (TSC). Using genetic modified mouse models, I am trying to reveal the molecular mechanism that leads to defects in the myelinated axon domain organization as is often observed in TSC patients.
Shi Q, Saifetiarova J, Taylor AM, Bhat MA (2018) mTORC1 Activation by Loss of Tsc1 in Myelinating Glia Causes Downregulation of Quaking and Neurofascin 155 Leading to Paranodal Domain Disorganization. Front. Cell. Neurosci. doi: 10.3389/fncel.2018.00201
Saifetiarova J, Shi Q, Paukert M, Komada M, Bhat MA (2018) Reorganization of Destabilized Nodes of Ranvier in βIV Spectrin Mutants Uncovers Critical Timelines for Nodal Restoration and Prevention of Motor Paresis. J. Neurosci. DOI: https://doi.org/10.1523/JNEUROSCI.0515-18.2018
Taylor AM, Shi Q, Bhat MA (2018) Simultaneous Ablation of Neuronal Neurofascin and Ankyrin G in Young and Adult Mice Reveals Age-Dependent Increase in Nodal Stability in Myelinated Axons and Differential Effects on the Lifespan. eNeuro DOI: https://doi.org/10.1523/ENEURO.0138-18.2018
Q. Shi, S. Viswanadhapalli, W. E. Friedrichs, C. Velagapudi, C. Szyndralewiez, S. Bansal, M.A. Bhat, G.G. Choudhury, and H. E. Abboud. Nox4 is a Target for Tuberin Deficiency Syndrome. Sci. Rep. 2018 doi: 10.1038/s41598-018-21838-4
Niu*, Q. Shi*, W. Zhang, Y. Shu, N. Yang, B, Chen, Q. Wang, X. Zhao, J. Chen, N. Cheng, X. Feng, J. Ji and P. Shen. Caspase-1 cleaves PPARγ for potentiating the pro-tumor action of TAMs. Nat. Comm. 2017, 3;8(1):766 * Equal contribution
Y. Yao*, Q. Shi*, B. Chen, Q. Wang, X. Li, L. Li, Y. Huang, J. Ji and P. Shen. Identification of caspase-6 as a new regulator of alternatively activated macrophages. J. Bio. Chem. 2016, 291 (33): 17450. * Equal contribution.
Q. Shi and J. X. Jiang. Connexin Arrests Cell Cycle Through Cytosolic Retention of an E3 Ligase. Mol. & Cell. Onco. 2016 Feb 18;3(2): e1132119.
Q.D. Zhao, S. Viswanadhapalli, P. Williams, Q. Shi, C. Tan, X. Yi, B. Bhandari, H.E. Abboud. NADPH Oxidase 4 Induces Cardiac Fibrosis and Hypertrophy Through Activating Akt/mTOR and NFκB Signaling Pathways. Circulation. 2015, 131(7): 643-655
Q. Shi, X. S. Yu, T. W. White, E.A. Bank, S. Gu and J. X. Jiang. Connexin Controls Cell-Cycle Exit and Cell Differentiation by Directly Promoting Cytosolic Localization and Degradation of E3 Ligase Skp2. Dev. Cell. 2015, 35(4):483-496.
Q. Shi, R. Padmanabhan, C. J. Villegas, S. Gu, and J. X. Jiang. Membrane Topological Structure of System N/A Neural Amino Acid Transporter SNAT4. J. Biol Chem.2011, Sep 14. 286: 38086-38094.
Q. Shi, E.A. Banks, X.S. Yu, S. Gu, J. Lauer, G.B. Fields, J.X. Jiang. Amino acid residue V362 plays a critical role in maintaining the structure of C-terminus of connexin 50 and in lens epithelial-fiber differentiation. J Biol Chem. 2010, 285(24):18415-18422.
T. Chen, Q. S. Wang, J. Cui, W. Yang, Q. Shi, J. G. Ji, P. P. Shen, Induction of apoptosis in mice liver by microcystin: a combined transcriptomic, proteomic and simulation strategy. Molecular & Cell Proteomics, 2005 (4):958-974. (Cover story)
Q. Shi, J. Cui, J. Zhang, F. X. Kong, Z. C. Hua, P. P. Shen, Expression modulation of multiple cytokines in vivo by cyanobacteria blooms extract from Taihu Lake, China. Toxicon 2004 (44):871-879.